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128818081
MEDIA - DMEM w/ 4.5 g/L glucose and sodium pyruvate w/out L-glutamine and phenol red - 6 x 500 mLq, CS
91% of respondents would recommend this to a friend
Item #:
128818081
XCD 559
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Dulbecco's Modified Eagle's Medium (DMEM) ensures improved stability and lower endotoxin content for superior results in cell culture.
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What Stands Out
High Glucose Content
Contains 4.5 g/L glucose for enhanced energy supply, ideal for culturing demanding cell lines requiring robust growth conditions.
Sodium Pyruvate Inclusion
Formulated with sodium pyruvate, offering additional energy support and protecting cells from oxidative stress during culture.
No L-Glutamine
Specifically designed without L-glutamine to provide researchers flexibility in supplementation, preventing issues related to L-glutamine degradation during storage.
Product Details
| Date First Available | April 27, 2017 |
| Manufacturer | Mediatech, Inc. |
| Brand Name | MEDIA |
| Item Form | Liquid |
| Container Type | Bottle |
| Dosage Form | Liquid |
| Number of Items | 6 |
| Item Weight | 9.4 lbs (4.26 kg) |
Who Should Buy?
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Cell Culture Labs
Ideal for laboratories specializing in mammalian cell cultures requiring high glucose concentrations and sodium pyruvate.
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Tissue Engineering
Suitable for applications in tissue engineering where precise nutrient control is necessary for cell growth.
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Research Studies
Beneficial for research settings focusing on metabolic studies involving cells in a glucose-rich environment.
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Glutamine-Dependent Cells
Not suitable for cell types that rely heavily on L-glutamine for survival and proliferation.
Product Description
MEDIA - DMEM w/ 4.5 g/L glucose and sodium pyruvate w/out L-glutamine and phenol red - 6 x 500 mLq, CS
Dietary Supplement Disclaimer
Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.
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Customer Questions & Answers
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Question:
What is DMEM and how is it used in cell culture?
Answer: DMEM, or Dulbecco's Modified Eagle Medium, is a widely used cell culture medium designed to support the growth and maintenance of various cell types. It contains essential nutrients like glucose and sodium pyruvate, which provide energy, and vitamins and minerals that facilitate cellular processes. In practical scenarios, DMEM is often utilized in laboratories for culturing fibroblasts, epithelial cells, and neuronal cells, ensuring optimal growth conditions are met. -
Question:
Why is L-glutamine excluded from this DMEM formula?
Answer: This specific DMEM formulation excludes L-glutamine to cater to experiments where glutamine supplementation is either not required or can introduce variability. Some cell lines perform better when they are supplemented with fresh glutamine on their own, allowing for more controlled conditions. Researchers often choose this medium for cells that are sensitive or show specific growth patterns in its absence. -
Question:
What are the benefits of using sodium pyruvate in cell culture media?
Answer: Sodium pyruvate serves as an important carbon source and a buffer in cell culture media, enhancing the metabolic flexibility of cells. It assists in cellular energy production and promotes better growth rates for many cell types. In practice, using DMEM with sodium pyruvate might be beneficial for cultures growing rapidly or under more stressful conditions, ensuring that cells thrive despite fluctuations in nutrient availability. -
Question:
Can this DMEM formulation support the growth of primary cells?
Answer: Yes, this DMEM formulation can support the growth of various primary cell types. Its balanced nutritional profile allows many cells to thrive. For instance, it can be effectively used for culturing primary neuronal or fibroblast cells. Proper conditions, including temperature and CO2 levels, should be maintained to optimize cell growth and viability. -
Question:
What is the significance of the glucose concentration in DMEM?
Answer: The glucose concentration in DMEM, set at 4.5 g/L, is significant as it provides an ample energy source for cellular metabolism. High glucose levels facilitate rapid growth and division of cells, especially in high-density cultures. This formulation is beneficial for studying highly proliferative cells like cancer cells, where energy demands are elevated. -
Question:
Is phenol red necessary in cell culture media?
Answer: Phenol red is often included in cell culture media as a pH indicator, providing visual feedback on medium changes. However, in specific applications, such as working with sensitive cells, its absence is preferred to avoid potential interference. Researchers may opt for formulations without phenol red to maintain the desired experimental conditions without the color change that might mislead pH assessments. -
Question:
How should DMEM be stored for optimal use?
Answer: DMEM should be stored in a cool environment, ideally at 2-8°C, and protected from light to prevent degradation of its components. Once opened, it’s advisable to utilize it within a specific timeframe to maintain its potency. For best practice, aliquoting the medium into smaller volumes can minimize contamination and allow for more flexible usage in experiments. -
Question:
What type of applications is this DMEM formulation suitable for?
Answer: This DMEM formulation is suited for a variety of applications, including basic research, drug testing, and toxicity assays. It is commonly used in studies involving cell migration and proliferation, making it ideal for cancer and regenerative medicine research. The absence of L-glutamine can also offer more controlled conditions specific to certain experimental designs. -
Question:
What kind of cells can be cultured effectively in DMEM?
Answer: DMEM can be used effectively for various cell lines, including HEK293, CHO, and several primary cell types. It supports both adherent and suspension cell growth, making it versatile for numerous experiments. Researchers often choose this medium for its reliability in cultivating cells that have highly specialized nutritional requirements or that exhibit rapid growth patterns. -
Question:
Where can I buy MEDIA - DMEM w 4.5 gL glucose and sodium pyruvate wout L-glutamine and phenol red - 6 x 500 mLq, CS in Saint Kitts and Nevis?
Answer: You can purchase MEDIA - DMEM w 4.5 gL glucose and sodium pyruvate wout L-glutamine and phenol red - 6 x 500 mLq, CS from Ubuy in Saint Kitts and Nevis. Ubuy offers a wide selection of laboratory products, making it a convenient option for researchers and professionals looking for high-quality media for their cell culture needs.
Nutritionist's Take
MEDIA - DMEM (Dulbecco's Modified Eagle Medium) with 4.5 g/L glucose and sodium pyruvate is primarily used in cell culture applications and not as a dietary product for human consumption. Nutritionists view its formulation, which lacks L-glutamine and phenol red, as suitable for specific laboratory settings where these ingredients may interfere with experimental outcomes. The high glucose content provides an energy source for cell metabolism, while sodium pyruvate can serve as an antioxidant and additional energy substrate. However, for those seeking nutritional supplementation or dietary health, this product is not a fit as it contains no vitamins, minerals, or other nutrients essential for human health. Additionally, the absence of L-glutamine may limit its utility in certain applications. Overall, while this product offers specific benefits in scientific research, it is not designed or recommended for human dietary use.
MEDIA L-Glutamine Editorial Review
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Features & Benefits
- Supports a variety of cells, including primary cultures.
- Contains 4.5 g/L glucose and sodium pyruvate.
- Formulated without L-glutamine and phenol red.
- Offers lower endotoxin content than major competitors.
- Improved stability due to Pyridoxine HCl substitution.
- Complete Certificate of Analysis available for every production lot.
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