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Item #:
92628330
Triton X-100 Lysis Buffer, 7.4 pH, 250mL
Item #:
92628330
XCD 205
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What Stands Out
Effective Cell Lysis
Triton X-100 lysis buffer efficiently breaks down cellular membranes, ensuring optimal release of proteins and nucleic acids for reliable analytical applications.
pH Stability
With a precise pH of 7.4, this buffer maintains physiological conditions, making it ideal for a range of biochemical experiments and ensuring reproducible results.
Generous Volume
The 250ml size provides ample supply for extensive laboratory work, reducing the frequency of reorder and enabling seamless experimentation for researchers.
Product Details
| Item Weight | 0.6 lbs (270 grams) |
Who Should Buy?
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Cell Biologists
Ideal for cell lysis, protein extraction, and maintaining cell viability in cell biology experiments.
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Protein Researchers
Useful for isolating proteins and analyzing protein structures in various biochemical studies.
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Academic Laboratories
Commonly employed in university labs for education and research focused on biochemistry and molecular biology.
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Non-Scientific Use
Not suitable for personal or non-research related applications due to its specialized chemical composition.
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Sensitive Organisms
May harm sensitive cell lines or organisms; alternative buffers might be better for delicate applications.
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Home Experiments
Not recommended for casual or home experiments due to potential safety and handling concerns for laypersons.
Product Description
Triton X-100 Lysis Buffer, 7.4 pH, 250mL
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is Triton X-100 Lysis Buffer used for?
Answer: Triton X-100 Lysis Buffer is primarily used in biological and biochemical applications to lyse cells and release cellular components. This buffer effectively disrupts cell membranes, allowing researchers to extract proteins, DNA, and other biomolecules for analysis. It's particularly beneficial in applications like protein solubilization, RNA extraction, and enzyme assays, facilitating more straightforward study and manipulation of cellular components. -
Question:
What is the pH of Triton X-100 Lysis Buffer?
Answer: The Triton X-100 Lysis Buffer has a pH of 7.4, which is close to physiological pH, making it suitable for maintaining the functionality of proteins and nucleic acids during lysis. This neutral pH helps to preserve the structure of biological molecules, ensuring that they remain active for downstream applications such as enzyme assays or Western blotting, where precise activity is crucial. -
Question:
How to prepare Triton X-100 Lysis Buffer?
Answer: To prepare Triton X-100 Lysis Buffer, dilute the required amount of Triton X-100 in a suitable buffer solution, such as PBS (phosphate-buffered saline), to achieve a desired concentration and pH of 7.4. Ensure to mix thoroughly and adjust the pH if necessary. This preparation process allows you to tailor the lysis buffer for specific applications in molecular biology or biochemistry, depending on the experimental requirements. -
Question:
Is Triton X-100 Lysis Buffer compatible with all cell types?
Answer: Yes, Triton X-100 Lysis Buffer is compatible with a variety of cell types, including mammalian, yeast, and bacterial cells. Its non-ionic surfactant properties allow it to effectively disrupt lipid membranes without denaturing proteins. This versatility makes it a preferred choice in labs working with different cell cultures and provides researchers the flexibility to adapt it to their specific experimental contexts. -
Question:
What is the volume of Triton X-100 Lysis Buffer provided in this product?
Answer: The product contains 250mL of Triton X-100 Lysis Buffer, providing ample solution for multiple experiments. This volume is typically sufficient for various applications, such as conducting cell lysis for protein extraction or DNA isolation in numerous assays. Therefore, it allows labs conducting routine analyses to effectively utilize the buffer for a consistent workflow. -
Question:
Can Triton X-100 Lysis Buffer be stored for long periods?
Answer: Yes, Triton X-100 Lysis Buffer can be stored for extended periods, typically at 4°C or at room temperature, protected from light. Stability is crucial for experiments that require a consistent lysis solution. Regularly check for any changes in appearance or precipitates before use, as this could indicate degradation, ensuring that your experiments yield reliable results. -
Question:
What precautions should be taken while using Triton X-100 Lysis Buffer?
Answer: While using Triton X-100 Lysis Buffer, it's important to wear appropriate personal protective equipment, such as gloves and goggles, due to the potential irritant nature of the chemical. Additionally, working in a well-ventilated area minimizes exposure to any vapors. Following these precautions ensures a safer lab environment while conducting critical experiments involving cell lysis and extraction processes. -
Question:
Is Triton X-100 Lysis Buffer suitable for protein assays?
Answer: Absolutely, Triton X-100 Lysis Buffer is highly suitable for protein assays, as it solubilizes proteins effectively without denaturing them. Its compatibility with various downstream applications, such as ELISA or Western blotting, makes it an essential tool in protein research. Researchers often prefer this buffer for its ability to maintain protein integrity, ensuring accurate assay outcomes and reliable data. -
Question:
Where can I buy Triton X-100 Lysis Buffer, 7.4 pH, 250mL in Saint Kitts and Nevis?
Answer: You can purchase Triton X-100 Lysis Buffer, 7.4 pH, 250mL from Ubuy, which offers a wide selection of scientific and laboratory products. Ubuy is known for its reliable service, providing customers easy access to research-grade materials suited for various experiments and applications. -
Question:
What are some applications of Triton X-100 Lysis Buffer?
Answer: Triton X-100 Lysis Buffer is widely used in applications such as cell lysis for protein extraction, RNA isolation, and enzyme activity assays. Its effective membrane disruption allows for successful cell component retrieval, making it a staple in molecular biology and biochemistry labs. Researchers utilize this buffer for its reliability, ensuring optimized sample preparation for various downstream analyses and experiments.
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Pros
- Effective cell lysis
- Optimal pH level
- High purity buffer
- Convenient size
- Versatile applications
Cons
- Pricey alternative
Product Price History
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XCD 205
Order now and get it around Sunday, July 05
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Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- Comprehensive range of biological buffers available.
- Designed for research, diagnostics, and drug development.
- Manufactured in an ISO 9001 and 13485 certified facility in the USA.
- Produced under stringent Good Manufacturing Practices.
- Quality control tests performed in certified clean room conditions.
- Ideal for protein research and molecular biology studies.