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Item #:
145284672
Phosphate Buffer, pH 7.4 [0.5 M] - 500 ml
85% of respondents would recommend this to a friend
Item #:
145284672
XCD 282
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What Stands Out
Optimal pH Stability
Maintains a precise pH of 7.4, crucial for biological experiments and cell culture applications, ensuring accurate and reproducible results every time.
High Concentration
With a 0.5 M concentration, this buffer offers enhanced buffering capacity, making it suitable for various biochemical and molecular biology applications.
Convenient Size
Available in a 500 ml bottle, it provides ample solution for multiple experiments, reducing the need for frequent re-purchasing and ensuring cost-effectiveness.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 8 ounces (226.8 grams) |
| Volume | 500 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.82 |
| Included Components | Bottle |
| Brand | eCon Lab Supply |
| Item Volume | 500 Milliliters |
| Package Information | Bottle |
Who Should Buy?
-
Biochemists
Essential for enzyme reactions where maintaining pH is critical for optimal activity and stability.
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Cell Culture
Ideal for maintaining physiological pH in cell culture media, promoting cell viability and growth.
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Laboratory Research
Suitable for various experiments requiring precise buffer conditions to ensure reproducibility and accuracy.
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Field Use
Not convenient for fieldwork where precise pH controls are difficult to manage in variable environments.
Product Description
Phosphate Buffer, pH 7.4 [0.5 M] - 500 ml
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Customer Questions & Answers
-
Question:
What is Phosphate Buffer, pH 7.4 used for?
Answer: Phosphate Buffer, pH 7.4 is commonly used in biological and biochemical experiments to maintain a stable pH environment. This stability is crucial in experiments involving enzymes, DNA/RNA isolation, and cell culture, as many biological processes are pH-sensitive. The buffer helps ensure that the pH remains consistent, optimizing conditions for reactions and preventing unwanted changes that could affect experimental outcomes. -
Question:
How do I prepare a 0.5 M Phosphate Buffer, pH 7.4?
Answer: To prepare a 0.5 M Phosphate Buffer, you need to mix specific amounts of monosodium phosphate and disodium phosphate. Typically, adjust the pH to 7.4 using a pH meter while adding each component gradually. This buffer concentration is ideal for most cell and molecular biology applications, allowing researchers to maintain appropriate pH levels for enzyme activity and cellular metabolism during experiments. -
Question:
Can Phosphate Buffer be used for protein stability?
Answer: Yes, Phosphate Buffer, pH 7.4 is widely used to stabilize proteins in biochemical studies. Many proteins have optimal activity around this pH, making it essential for maintaining their structure and function during experiments. Researchers often use this buffer in protein purification and storage to prevent denaturation and aggregation, ensuring that the proteins remain active for downstream applications. -
Question:
What is the significance of the pH level in Phosphate Buffer?
Answer: The pH level of 7.4 in Phosphate Buffer is significant because it closely resembles physiological conditions in the human body, making it an ideal choice for biological experiments. By mimicking these natural conditions, researchers can ensure that their experimental results are more applicable to in vivo systems. This is particularly important in pharmacology and disease modeling studies, where maintaining physiological relevance is crucial for accurate data interpretation. -
Question:
What applications is a 0.5 M Phosphate Buffer suitable for?
Answer: A 0.5 M Phosphate Buffer, pH 7.4 is suitable for various applications, including enzyme assays, DNA and RNA manipulation, and cell culture work. Its ability to maintain a consistent pH environment makes it essential in laboratory settings, particularly when conducting experiments that require precise conditions for optimal results. This buffer is also used in diagnostic testing and research involving cellular behavior under controlled pH levels. -
Question:
Is Phosphate Buffer toxic or safe to handle?
Answer: Phosphate Buffer, pH 7.4, is generally considered safe to handle and is not classified as toxic. However, as with any laboratory chemical, it is important to follow standard safety protocols such as wearing gloves, goggles, and lab coats. Familiarity with Material Safety Data Sheets (MSDS) helps ensure safe handling practices. This safety profile makes it suitable for educational purposes, allowing students and researchers to work confidently during experiments. -
Question:
How should Phosphate Buffer be stored?
Answer: Phosphate Buffer, pH 7.4, should be stored in a tightly sealed container, preferably in a cool and dry place, away from direct sunlight. Proper storage conditions help preserve its effectiveness, preventing any pH changes over time. Laboratory environments that maintain consistent temperature and humidity levels are ideal, ensuring that the buffer remains stable and ready for use when needed for experiments. -
Question:
Can Phosphate Buffer be used in water-based experiments?
Answer: Yes, Phosphate Buffer is water-soluble and can be effectively used in water-based experiments. It is particularly useful in applications that involve aqueous solutions, such as cell culture and biochemical assays. Its compatibility with water makes it suitable for a range of experimental setups, allowing researchers to efficiently conduct various analyses without altering the solubility or reactivity of their materials. -
Question:
What concentration of Phosphate Buffer is typically used in cell culture?
Answer: In cell culture, a 0.5 M Phosphate Buffer, pH 7.4 is commonly used to maintain the stability of the pH during cell growth and maintenance. This concentration effectively supports cellular metabolism and growth conditions. Utilizing this buffer helps minimize fluctuations in pH that could adversely affect cell health, ensuring optimal growth and proliferation for various types of cultured cells in research and therapeutic settings. -
Question:
Where can I buy Phosphate Buffer, pH 7.4 [0.5 M] - 500 ml?
Answer: You can purchase Phosphate Buffer, pH 7.4 [0.5 M] - 500 ml through Ubuy. Ubuy is a reliable online platform that offers a variety of laboratory products, making it easy to find what you need for your experiments. The platform provides detailed product descriptions and specifications, ensuring you select the right buffer for your specific applications.
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Features & Benefits
- Comprehensive range of biological buffers available.
- Ideal for research, diagnostics, and drug development.
- Manufactured in an ISO 9001 and 13485 certified facility.
- Each lot follows stringent Good Manufacturing Practices.
- Produced in certified clean room conditions for quality.
- Not for human or animal clinical/therapeutic use.
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