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Item #:
97963595
Tris.HCl (AR Grade, 5 kg)
Item #:
97963595
XCD 959
Price Details
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AR Grade chemicals are a very high grade suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.
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What Stands Out
High Purity
Tris.HCl AR Grade ensures exceptional purity levels, suitable for laboratory applications and biochemical research, offering confidence in experimental results without contamination.
Bulk Quantity
The 5 kg packaging is ideal for institutions and labs needing large volumes, reducing the frequency of reorders while maximizing value and convenience.
Versatile pH Buffer
This product serves as a reliable pH buffer in various biological assays, enhancing experimental accuracy and maintaining optimal conditions for enzyme reactions and electrophoresis.
Product Details
| Item Weight | 11.02 lbs (5 kg) |
Who Should Buy?
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Biochemistry Labs
Ideal for buffer preparation and enzyme assays in biochemical research and analysis.
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Molecular Biology
Used in protocols for DNA and RNA isolation, purification, and amplification techniques.
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Educational Institutions
Perfect for universities and colleges conducting experiments in chemistry or life sciences courses.
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Basic Household Use
Not suitable for general home use or non-research applications due to its specialized nature.
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Non-Scientific Fields
Not appropriate for users in fields without a chemistry or biochemical focus who do not require buffers.
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Small Scale Projects
Purchasing 5 kg may be excessive for individuals or projects requiring only minimal amounts for experimentation.
Product Description
Tris.HCl (AR Grade, 5 kg)
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Customer Questions & Answers
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Question:
What is Tris.HCl AR Grade used for?
Answer: Tris.HCl AR Grade, commonly referred to as Tris buffer, is widely utilized in biochemical and molecular biology research. Its primary function is to maintain a stable pH in biological experiments. Tris buffer is particularly valuable during electrophoresis, helping to ensure consistent results by stabilizing the pH of the running buffer. -
Question:
What is the composition of Tris.HCl AR Grade?
Answer: Tris.HCl AR Grade consists of Tris (tris(hydroxymethyl)aminomethane) and hydrochloric acid, forming a buffer solution that is effective in maintaining pH levels within the range of 7 to 9. The purity of AR Grade is essential for laboratory applications as it minimizes interference in sensitive experiments, making it ideal for use in protein studies and DNA analyses. -
Question:
How do I prepare a Tris buffer solution?
Answer: To prepare a Tris buffer solution, dissolve an appropriate amount of Tris in distilled water, then adjust the pH using HCl. Typically, a 1 M solution is prepared and adjusted to the desired pH. This solution is crucial for various laboratory applications, such as running gel electrophoresis, where pH stability ensures the reliable migration of biomolecules. -
Question:
Is Tris.HCl AR Grade safe to use?
Answer: Yes, Tris.HCl AR Grade is generally safe when handled appropriately. However, as with any chemical, it is advisable to use personal protective equipment, such as gloves and goggles, to avoid contact with skin or eyes. Proper lab practices should always be followed to ensure a safe working environment while using this reagent. -
Question:
What storage conditions are recommended for Tris.HCl AR Grade?
Answer: Tris.HCl AR Grade should be stored in a cool, dry place, away from direct sunlight. It is best kept in a tightly sealed container to prevent moisture absorption. Proper storage of chemicals is crucial to maintaining their efficacy and longevity for future use in laboratory experiments. -
Question:
Can Tris.HCl AR Grade be used in DNA extraction processes?
Answer: Absolutely! Tris.HCl AR Grade is frequently used in DNA extraction protocols. Its ability to maintain a stable pH helps in preserving the integrity of DNA during extraction and purification steps, making it essential in molecular biology labs focused on genomics and genetic analysis. -
Question:
What are some alternatives to Tris.HCl buffer?
Answer: Alternative buffers to Tris.HCl include phosphate-buffered saline (PBS) and HEPES. Each buffer has its own pH range and use cases, such as for enzyme reactions or cell culture. The choice of buffer often depends on the specific requirements of your experiment, such as the desired pH stability and ionic strength. -
Question:
How does Tris.HCl affect protein stability?
Answer: Tris.HCl helps maintain protein stability by providing a consistent pH, which is crucial for enzymatic activity and protein folding. Many proteins are sensitive to pH changes, and using a Tris buffer can help ensure that proteins remain active during experimental procedures, making it vital in biochemical assays. -
Question:
Where can I buy Tris.HCl AR Grade, 5 kg in Saint Kitts and Nevis?
Answer: You can purchase Tris.HCl AR Grade, 5 kg from Ubuy. They offer a reliable selection of laboratory chemicals, including high-quality buffers like Tris.HCl, suitable for various scientific applications. Ubuy ensures proper sourcing and delivery of reagents directly to your location.
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Pros
- High purity for reliable results
- Versatile in biochemical applications
- Stability across various pH levels
- Cost-effective for laboratory use
- Large quantity suitable for research
Cons
- Occasional clumping in storage
Product Price History
Important information
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XCD 959
Order now and get it around Saturday, July 04
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Secured transaction
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Features & Benefits
- CAS Number: 1185-53-1
- Molecular Weight: 157.64
- Appearance: White crystalline
- Assay: ≥99.5% AR grade
- Storage Condition: Room temperature
- Ideal for laboratory and analytical applications.